TO STUDY THE OXIDATIVE STRESS AND ANTIOXIDANTS STATUS IN DIABETES MELLITUS WITHOUT COMPLICATION

Abstract

Objective: To study the oxidative stress and Antioxidants status in Diabetes mellitus without complication

Methods: Twenty Two patients of type II diabetes mellitus without complication and Fourteen healthy, non-diabetic, age and sex matched individuals were selected as the controls. Oxidative stress markers (NO, LPO) and antioxidant markers (GSH, SOD) measured by appropriate methods in both the groups.

Results: In type II diabetes mellitus without complication Free Radicals measurement were LPO MDA (23.95±0.85 μmol/L) and NO (702.1±19.1 nmol/L) and in healthy, non-diabetic, age and sex matched individuals Free Radicals measurement were LPO MDA (17.05±2.10) and NO (527.7±16.0) with p value 0.007 and <0.001 respectively which is statistically significant. Where as In type II diabetes mellitus without complication antioxidant measurement GSH (78.3±2.77μmol/L)and SOD (891.6±52.3 U/L) and in healthy, non-diabetic, age and sex matched individuals antioxidant measurement were GSH (96.2±1.69) and SOD (1323.2 ± 105) with p value <0.001 and 0.002 respectively which is statistically significant.

Conclusion: The current study suggests that the oxidative stress may have role in the pathogenesis of diabetes, as indicated by the increased levels of lipid peroxidation product malondialdehyde in patients than age and sex matched healthy controls. The decreased levels of the Glutathione peroxidase and Superoxide dismutase in the antioxidant enzyme mechanisms indicates that the anti-oxidative enzyme system gets impaired more diabetic patients.