BACTERIOLOGICAL ANALYSIS AND RESISTANCE PATTERN AMONG VARIOUS CULTURE ISOLATES FROM NEONATAL SEPTICEMIA AT TERTIARY CARE HOSPITAL OF AHMEDABAD

Abstract

Introduction: Septicemia remains a significant cause of morbidity and mortality in the newborn. Shortly after the introduction of potent and broad-spectrum antibiotics, the emergence of resistant strains became a major problem in various Intensive care units. So, determination of bacterial etiology and antibiotic susceptibility patterns of isolates from septicemia in Neonatal intensive care units (NICU) is now crucial to abate neonatal mortality. This study was under taken to know the bacterial etiology of septicemia in neonate and antibiotic susceptibility pattern of the isolates.

Materials and Methods: During Jan-2011 to June -2011, 626 Blood samples were collected aseptically from newborns admitted with sepsis in NICU, Sheth Vadilal Sarabhai General Hospital, Ahmadabad and processed by standard conventional method. Antibiotic susceptibility pattern of isolates was studied by Kirby Bauer Disc diffusion technique.

Results: Total 626 samples were received during the study period of which 107 (17.09%) samples were found to be positive. Out of 107 isolated organisms 59 (55.14%) were Klebsiella sp., 11 (10.28%) Staphylococcus aureus, 10 (9.34%) Escherichia coli, 9 (8.41%) Coagulase negative staphylococci, 7 (6.54%) Pseudomonas aeruginosa, 5 (4.67%) Enterobacter sp.5, 4(0.63%) Enterococcus sp., 1 (0.93%) Acinetobacter baumanii and 1 (0.93%) Proteus-mirabilis. Majority of organisms isolated were resistant to commonly used antibiotics. Imipenem showed 100% sensitivity for gram negative organisms. Methicillin resistance was found in 9.01% Staphylococcus aureus. Isolated all Enterococcus sp. were sensitive to vancomycin and high level gentamycin.

Conclusion: Multi-drug resistance organisms were isolated from septicemia in neonates. This study would guide the clinicians to formulate appropriate treatment strategy as well as to take various preventive measures which ultimately would help to decrease neonatal mortality.