ANTIBIOTIC RESISTANCE PATTERN IN PSEUDOMONAS AERUGINOSA SPECIES ISOLATED AT A TERTIARY CARE HOSPITAL, AHMADABAD

Abstract

Introduction: Pseudomonas aeruginosa (Ps.aeruginosa) is one of the important bacterial pathogens isolated from various samples. Despite advances in medical and surgical care and introduction of wide variety of antimicrobial agents against having anti-pseudomonal activities, life threatening infection caused by Ps. aeruginosa continues to cause complications in hospital acquired infections. Several different epidemiological studies indicate that antibiotic resistance is increasing in clinical isolates.

Material and Method: This study was conducted during April 2009 to april 2010. During this period total of 630 samples were tested, in which 321 samples showed growth of bacteria. Out of 321 samples, 100 clinical isolates of Pseudomonas aeruginosa were isolated. The samples were selected on the basis of their growth on routine MacConkey medium which showed lactose Non-fermenting pale colonies which were oxidase test positive and on Nutrient agar pigmented and non-pigmented colonies with oxidase positive. Antimicrobial susceptibility of all the isolates was performed by the disc-diffusion (Modified-Kirby Baur disc diffusion method) according to CLSIs guidelines.

Result: In present study, maximum isolates of Ps. aeruginosa isolated from various samples are resistant to tobramycin (68%) followed by gentamycin (63%), piperacillin (50%), ciprofloxacin (49%) and ceftazidime (43%).

Conclusion: To prevent the spread of the resistant bacteria, it is critically important to have strict antibiotic policies while surveillance programmes for multidrug resistant organisms and infection control procedures need to be implemented.