ISOLATION OF ASPERGILLUS SPECIES FROM SPUTUM SAMPLES: A STUDY CONDUCTED IN A TERTIARY CARE HOSPITAL, AHMEDABAD

Abstract

Introduction: In the last three decades, fungal infection has become important cause respiratory tract infection. The increase in frequency is mainly due to intensive cytotoxic therapy, greater use of broad spectrum antibiotics, corticosteroids and Immuno-suppresants.

Material and Method: Sputum Samples were collected from june 2005 to june 2006.The samples were subjected to direct microscopy using Gram staining, KOH wet mounts, India ink preparations depending on the type of specimen and the suspected infection in the patient. Fungal culture was done on Sabouraud dextrose agar, with and without chloramphenicol, Czapek^`s solution agar and malt extract agar.

Result: This study was undertaken to determine isolation of Aspergillus species from sputum samples. 61% of sputum specimens were culture positive and 39% were negative among cases of chronic respiratory diseases. 29% of 100 samples are aspergillus spp. while 32% of positive culture are other than aspergillus spp. Highest number of male & female patients were from 31-40 years of age and then gradually decrease in number in 21-30 and 41-50 years of age group.

Conclusion: An increase in rate of infection occurs during a period of building construction, particularly in zones surrounding hospitals.